Structural Basis of Subtilase Cytotoxin SubAB Assembly
نویسندگان
چکیده
منابع مشابه
Escherichia coli Subtilase Cytotoxin
Subtilase cytotoxin (SubAB) is the prototype of a new AB(5) toxin family produced by a subset of Shiga toxigenic Escherichia coli (STEC) strains. Its A subunit is a subtilase-like serine protease and cytotoxicity for eukaryotic cells is due to a highly specific, single-site cleavage of BiP/GRP78, an essential Hsp70 family chaperone located in the endoplasmic reticulum (ER). This cleavage trigge...
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Subtilase cytotoxin (SubAB) is the prototype of a new family of AB(5) cytotoxins produced by Shiga-toxigenic Escherichia coli. Its cytotoxicity is due to its capacity to enter cells and specifically cleave the essential endoplasmic reticulum chaperone BiP. Previous studies have shown that intraperitoneal injection of mice with purified SubAB causes a pathology that overlaps with that seen in hu...
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Table 1 Designations, targets, and positions of primers for PCR analysis and Southern blot hybridization Primer Target Sequence (5′ – 3′) GenBank accession number Reference saaDF saa 5′-CGT GAT GAA CAG GCT ATT GC-3′ AF399919 [26] saaDR saa 5′-ATG GAC ATG CCT GTG GCA AC-3′ AF399919 [26] subAB-V-for subAB 5′-CTT CCC TCA TTG CCT CAC G-3′ AY258503 This study subAB-V-rev subAB 5′-GGC TGG CCT GTT GTG...
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Subtilase cytotoxin (SubAB) is an AB(5) cytotoxin produced by some strains of Shiga-toxigenic Escherichia coli. The A subunit is a subtilase-like serine protease and cleaves an endoplasmic reticulum chaperone, BiP, leading to transient inhibition of protein synthesis and cell cycle arrest at G(1) phase. Here we show that SubAB, but not the catalytically inactive mutant SubAB(S272A), induced apo...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2013
ISSN: 0021-9258
DOI: 10.1074/jbc.m113.462622